objective zeiss fluar 2.5x Search Results


ec plan neofluar  (Carl Zeiss)
96
Carl Zeiss ec plan neofluar
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Ec Plan Neofluar, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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inverted fluorescence microscope  (Carl Zeiss)
99
Carl Zeiss inverted fluorescence microscope
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Inverted Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Inverted+microscope+Axio+Observer+3/pmc10144907-343-25-28
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zeiss axio observer 7  (Carl Zeiss)
98
Carl Zeiss zeiss axio observer 7
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Zeiss Axio Observer 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Inverted+microscope+Axio+Observer+7/pm38537433-65-23-23
Average 98 stars, based on 1 article reviews
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gaasp photomultipliers h10770pa-40 sel  (Hamamatsu)
90
Hamamatsu gaasp photomultipliers h10770pa-40 sel
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Gaasp Photomultipliers H10770pa 40 Sel, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/photomultiplier+tube+hamamatsu+r928/pmc05909709-75-12-14
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stereomicroscope  (Carl Zeiss)
97
Carl Zeiss stereomicroscope
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Stereomicroscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Stereomicroscope+SteREO+Discovery%2EV8+with+stand+300/pmc11210988-71-11-17
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axio observer z1 microscope  (Carl Zeiss)
96
Carl Zeiss axio observer z1 microscope
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Axio Observer Z1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Microscope+stand+Axio+Observer+5/bio_rxiv__2022__11__21__517317-122-24-27
Average 96 stars, based on 1 article reviews
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tissue scanner zeiss axioskop plan neofluar zeiss objectives  (Carl Zeiss)
91
Carl Zeiss tissue scanner zeiss axioskop plan neofluar zeiss objectives
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Tissue Scanner Zeiss Axioskop Plan Neofluar Zeiss Objectives, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Objective+EC+Plan-Neofluar+2%2E5x%2F0%2E085+M27/pmc13619062-48-16-18
Average 91 stars, based on 1 article reviews
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ip-s  (Nanoscribe GmbH)
90
Nanoscribe GmbH ip-s
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Ip S, supplied by Nanoscribe GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/ip+s/bio_rxiv__2024__11__05__622167-344-8-9
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zeiss v16 axio zoom  (Carl Zeiss)
97
Carl Zeiss zeiss v16 axio zoom
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Zeiss V16 Axio Zoom, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Zoom+microscope+Axio+Zoom%2EV16/pm41792134-451-19-19
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zeiss axiolab polarising microscope  (Carl Zeiss)
96
Carl Zeiss zeiss axiolab polarising microscope
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
Zeiss Axiolab Polarising Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Microscope+Axiolab/10__1016_slash_j__conbuildmat__2024__135700-56-14-14
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25x water  (Nikon)
99
Nikon 25x water
Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A <t>1.25x</t> objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.
25x Water, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/objective+zeiss+fluar+2%2E5x/Objectives/pm37875964-121-12-20
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Image Search Results


Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A 1.25x objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.

Journal: Scientific reports

Article Title: Optogenetics design of mechanistically-based stimulation patterns for cardiac defibrillation.

doi: 10.1038/srep35628

Figure Lengend Snippet: Figure 1. Simultaneous all-optical map and control of cardiac conduction pathway. (a) Scheme of the wide-field fluorescence macroscope consisting of a LED at a wavelength centered at 625 nm for excitation of the voltage-sensitive dye and a CW laser at 473 nm for ChR2 activation. The system is provided with a random access scanning head comprised of two orthogonally mounted acousto-optical deflectors (AOD x-y). A 1.25x objective is used for imaging and stimulation of the whole mouse heart, a 20x objective is used to focus the fluorescence signal into a central portion (100 × 100 pixel) of a sCMOS sensor operating at a frame rate of 2 kHz. (b) Electrocardiographic recording of a ChR2-expressing heart, showing sinus rhythm with a frequency of 3 Hz. Blue arrowheads represent the ChR2 stimulation performed at 5 Hz (laser power at the sample 4.9 mW, illumination time 5 ms). The orange line represents the LED illumination. (c) Fluorescence image (F0) of a ChR2-expressing heart stained with voltage sensitive dye (left). Scale bar of 2 mm in yellow. Six representative frames of optical mapping (ΔF/F0) recorded from the same heart. The electrical activation is reported in red and the baseline in cyan. On the right, corresponding color-scaled isochronal map of the action potential reporting the voltage activation time per pixel. (d) On the left, fluorescence image (F0) of a ChR2-expressing heart stimulated in the apex either with an electrode (red spot, above) or with ChR2 activation with the blue laser (blue spot, below). The green frame represents the AODs working-field on the sample. Corresponding optical mapping frames (ΔF/F0) recorded from the same heart are reported next. On the right, corresponding color-scaled isochronal map of the action potential reporting the activation time per pixel.

Article Snippet: The whole mouse heart was excited in wide-field configuration using a 1.25x objective (EC Plan-Neofluar 1.25x/0.03 M27, Carl Zeiss Microscopy) and a LED operating at a wavelength centered at 625 nm (M625L3, Thorlabs) followed by a band-pass filter at 640/40 nm (FF01640/40-25, Semrock).

Techniques: Control, Fluorescence, Activation Assay, Imaging, Expressing, Staining